Prescription drug abuse became a major problem today. According to DEA data, the number of Methadone drug items seized by law enforcement increased from 2,865 in 2001 to 10,361 in 2007, suggesting that Methadone contributed to rise in overdose deaths. In order to control this, methadone abusers must be detected using one step Methadone test. This test is very much preferred as it is simple and detects the methadone abuse within minutes.
Methadone test procedure: One step Methadone test is a ‘dip and read’ type test that is simple to administer to detect the methadone content in urine specimen. This test can be performed without using any other instruments. This test detects the elevated levels of Methadone by using the reaction of antibody to its antigen.
Prior to testing, allow the test card and urine specimen to reach room temperature (15-30° C). Remove the test card from the pouch and discard the moisture absorbent packet. Immerse the test strip in urine specimen for 10 to 15 seconds, with arrows pointing towards the specimen. While immersing the test strip, do not cross the maximum (MAX) line on strip into the urine specimen. Place the test card on a non absorbent flat surface, set the timer and wait for the lines to appear. Take the results at five minutes and do not take after ten minutes.
Interpretation: One step Methadone test results are 95% to 98% accurate. This test yields positive results when the content of Methadone in urine is more than 300 ng/ml. For a positive result, a red line appears in the control (C) region and no line appears in the test (T) region. For a negative result, one red line can be seen in the control region and another red or pink line appears in the test region. When the result is invalid, line in the control region fails to appear. The most common reasons for invalid results are insufficient specimen volume or incorrect procedural techniques.
The one step Methadone test provides initial analytical test results. In order to obtain a confirmed analytical result, a more specific alternate chemical method, Gas Chromatography/Mass Spectrometry (GC/MS) must be used to confirm the abuse, particularly after positive results in the initial tests. The cut-off levels for Methadone test recommended are 300 ng/ml for initial test and 250 ng/ml for confirmation test.
In order to overcome the rising prescription drug abuse, one should detect and control the Methadone abuse. And one step Methadone test is an important tool in detecting the abusers. This test is simple and provides accurate results which are set to SAMHSA cut off levels.
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